Journal: Antioxidants
Article Title: DNA Damage in Human Amniotic Cells: Antigenotoxic Potential of Curcumin and α -Lipoic Acid
doi: 10.3390/antiox10071137
Figure Lengend Snippet: ( a ) Percentage of DNA fragmentation (ordinate) in amniotic cells after different hours of exposure (abscissa) to H 2 O 2 , H 2 O 2 and LA (co-exposure), H 2 O 2 and curcumin (Cur) (co-exposure) and LA plus Cur and H 2 O 2 . The black bars are negative control (NC); the white bars are positive control (10 mM H 2 O 2 ); the light grey bars are 10 mM H 2 O 2 + 200 µM α-lipoic acid (LA 1 + H 2 O 2 ); the dark grey bars are 10 mM H 2 O 2 + 40 µM curcumin (Cur 2 + H 2 O 2 ); the grey dotted bars are 200 µM α-lipoic acid plus 40 µM curcumin with 10 mM H 2 O 2 co-treatment (LA 1 + Cur 2 + H 2 O 2 ). DNA fragmentation index was calculated as follows: DFI = (number of green nuclei/number of total nuclei) × 100). Values with different letters differ with statistical significance (ANOVA). p ≤ 0.05. ( b ) Amniotic cells with fragmented DNA (green) and intact DNA (blue), after H 2 O 2 treatment, detected with Nikon E600 fluorescence microscope.
Article Snippet: Morphological observations were performed using an inverted optical microscope (Optika XDS-3LT trinocular inverse microscope, Optika, Ponteranica, Italy) with 400× magnification.
Techniques: Negative Control, Positive Control, Fluorescence, Microscopy